from a single amino acid sequence
file
Instructions:
-
Step 1:
-
Choose File/Open/.aa file to open a dialog
box (Fig. 1) for input.
Fig. 1 |
-
Step 2:
-
Enter the name of the file containing the amino acid
sequence in the field of ".aa FileName", or use the "Browse ..." button
to select it. Then provide the amino acid sequence range of each transmembrane
(TM) helix in the protein. You can input these helices' beginning and ending
sequence numbers with a file (See Fig. 2a) or type them manually (Fig.
2b). (For manual input, the maximal number of helices is currently set
to 30.) For a detail description of the files .aa,
and .helixseq , see Appendix A and
B.
Fig. 2a |
Fig. 2b |
-
Step 3:
-
Choose OK, VHMPT will draw a schematic 2D membrane
topology for the protein (e.g., Fig. 3).
Fig. 3 |